Effect of asbestos exposure on differentiation and function of cytotoxic T lymphocytes

Table 3 Functional properties of human CD8⁺ T cell line following long-term exposure to asbestos fibers

Parameters	EBT-8-Org	EBT-8-CH5 or/and EBT-8-CH30	Significance
Granzyme B (MFI)	++ or +	+ or ++	N.S.
Perforin⁺ (%)	++	+	p < 0.01
IFN-γ⁺ (%)	++ or +	+ or ++	N.S.
Production of IFN-γ	++	+	p < 0.0001
Degranulation (%)	+	+	N.S.

MFI mean fluorescence intensity, N.S. not significant
The human CD8+ T cell line was then cultured in the absence or presence of CH asbestos at low or middle concentrations of 5 or 30 μg/ml for 1 to 2 months, with cell groups being referred to as EBT-8-Org, EBT-8-CH5, and EBT-8-CH30, respectively

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