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Table 2 Results of factorial analysis for the relative weight of uterus treated with combination of BPA,NP and DES(n = 10)

From: Analysis of individual and combined estrogenic effects of bisphenol, nonylphenol and diethylstilbestrol in immature rats with mathematical models

group BMDL10 LOAEL
uterus/body weight ratio (g/kg, %) F Value P-Value uterus/body weight ratio (g/kg, %) F Value P-Value
Variance source
 Blank control 0.79 ± 0.08 0.79 ± 0.08
 vehicle control 0.75 ± 0.14 0.75 ± 0.14
Main effect
 BPA 0.80 ± 0.20 3.65 0.06 0.95 ± 0.23 11.87 P < 0.05
 NP 0.79 ± 0.22 1.00 0.32 0.93 ± 0.18 1.71 0.20
 DES 0.85 ± 0.19 0.72 0.40 0.93 ± 0.16 5.57 P < 0.05
Interaction
 BPA + NP 0.95 ± 0.27 1.07 0.31 1.00 ± 0.19 1.09 0.30
 BPA + DES 0.87 ± 0.21 0.12 0.73 1.08 ± 0.25 0.003 0.96
 NP + DES 0.80 ± 0.17 1.07 0.31 0.93 ± 0.12 2.69 0.11
 BPA + NP + DES 0.92 ± 0.23 0.01 0.911 1.06 ± 0.11 0.49 0.49
  1. ANOVA was used to test the statistical significance for the main effect and interaction. “P < 0.05” indicated statistically significant main effect for individula EDCs or interactions among EDCs
  2. BPA Bisphenol A; NP Nonylphenol, DES Diethylstilbestrol, BMDL Benchmark dose lower confidence limit,LOAEL Lowest observed adverse effect level